Cloning and characterization of squalene synthase gene from Poria cocos and its up-regulation by methyl jasmonate.
World J Microbiol Biotechnol
; 30(2): 613-20, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-24030169
ABSTRACT
Squalene synthase (SQS) catalyzes the condensation of two molecules of farnesyl diphosphate to give presqualene diphosphate and the subsequent rearrangement to form squalene. The gene encoding squalene synthase was cloned from Poria cocos by degenerate PCR and inverse PCR. The open reading frame of the gene is 1,497 bp, which encodes 499 amino acid residues. A phylogenetic analysis revealed that P. cocos SQS belonged to the fungus group, and was more closely related to the SQS of Ganoderma lucidum than other fungi. The treatment of P. cocos with methyl jasmonate (MeJA) significantly enhanced the transcriptional level of P. cocos sqs gene and the content of squalene in P. cocos. The transcriptional level of sqs gene was approximately fourfold higher than the control sample and the squalene content reached 128.62 µg/g, when the concentration of MeJA was 300 µM after 72 h induction.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Esqualeno
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Farnesil-Difosfato Farnesiltransferase
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Regulação Enzimológica da Expressão Gênica
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Regulação Fúngica da Expressão Gênica
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Ciclopentanos
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Oxilipinas
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Poria
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Acetatos
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article