Multivalent choline dendrimers increase phagocytosis of Streptococcus pneumoniae R6 by microglial cells.
Chemotherapy
; 59(2): 138-42, 2013.
Article
em En
| MEDLINE
| ID: mdl-24051739
ABSTRACT
BACKGROUND:
Pneumococcal virulence factors common to all serotypes, such as choline-binding proteins (CBPs), are promising therapeutic targets in pneumococcal infections. We studied the effect of a choline dendrimer with maximized binding affinity/specificity for CBPs on microglia-mediated pneumococcal phagocytosis.METHODS:
Pneumoccocal cultures were exposed to dendrimers containing 8 choline end groups or amino groups as controls, either from the beginning of bacterial growth or at the late exponential phase. The effect of long/short co-incubation was assessed in terms of bacterial morphological changes and increase in bacterial uptake by primary microglial cultures.RESULTS:
Inhibiting CBPs by micromolar concentrations of a choline dendrimer caused the formation of long pneumococcal chains that were readily phagocytosed by microglia. Enhanced phagocytosis was dendrimer dose-dependent. Long bacteria-dendrimer co-incubation (14 h) resulted in a higher bacterial uptake than short co-incubation (2 h; p < 0.001).CONCLUSIONS:
Multivalent dendrimers containing choline end groups are promising antimicrobial agents for the management of pneumococcal diseases.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fagocitose
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Streptococcus pneumoniae
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Colina
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Microglia
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Dendrímeros
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Anti-Infecciosos
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article