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Reactivation of NCAM1 defines a subpopulation of human adult kidney epithelial cells with clonogenic and stem/progenitor properties.
Buzhor, Ella; Omer, Dorit; Harari-Steinberg, Orit; Dotan, Zohar; Vax, Einav; Pri-Chen, Sara; Metsuyanim, Sally; Pleniceanu, Oren; Goldstein, Ronald S; Dekel, Benjamin.
Afiliação
  • Buzhor E; Pediatric Stem Cell Research Institute, Safra Children's Hospital, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ramat-Gan, Israel; Sheba Center for Regenerative Medicine, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ra
  • Omer D; Pediatric Stem Cell Research Institute, Safra Children's Hospital, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ramat-Gan, Israel; Sheba Center for Regenerative Medicine, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ra
  • Harari-Steinberg O; Pediatric Stem Cell Research Institute, Safra Children's Hospital, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ramat-Gan, Israel; Sheba Center for Regenerative Medicine, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ra
  • Dotan Z; Department of Urology, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ramat-Gan, Israel.
  • Vax E; Pediatric Stem Cell Research Institute, Safra Children's Hospital, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ramat-Gan, Israel; Sheba Center for Regenerative Medicine, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ra
  • Pri-Chen S; Pediatric Stem Cell Research Institute, Safra Children's Hospital, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ramat-Gan, Israel; Maurice and Gabriela Goldschleger Eye Research Institute, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel A
  • Metsuyanim S; Pediatric Stem Cell Research Institute, Safra Children's Hospital, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ramat-Gan, Israel; Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
  • Pleniceanu O; Pediatric Stem Cell Research Institute, Safra Children's Hospital, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ramat-Gan, Israel; Sheba Center for Regenerative Medicine, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ra
  • Goldstein RS; Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
  • Dekel B; Pediatric Stem Cell Research Institute, Safra Children's Hospital, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ramat-Gan, Israel; Sheba Center for Regenerative Medicine, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Ra
Am J Pathol ; 183(5): 1621-1633, 2013 Nov.
Article em En | MEDLINE | ID: mdl-24055371
The nephron is composed of a monolayer of epithelial cells that make up its various compartments. In development, these cells begin as mesenchyme. NCAM1, abundant in the mesenchyme and early nephron lineage, ceases to express in mature kidney epithelia. We show that, once placed in culture and released from quiescence, adult human kidney epithelial cells (hKEpCs), uniformly positive for CD24/CD133, re-express NCAM1 in a specific cell subset that attains a stem/progenitor state. Immunosorted NCAM1(+) cells overexpressed early nephron progenitor markers (PAX2, SALL1, SIX2, WT1) and acquired a mesenchymal fate, indicated by high vimentim and reduced E-cadherin levels. Gene expression and microarray analysis disclosed both a proximal tubular origin of these cells and molecules regulating epithelial-mesenchymal transition. NCAM1(+) cells generated clonal progeny when cultured in the presence of fetal kidney conditioned medium, differentiated along mesenchymal lineages but retained the unique propensity to generate epithelial kidney spheres and produce epithelial renal tissue on single-cell grafting in chick CAM and mouse. Depletion of NCAM1(+) cells from hKEpCs abrogated stemness traits in vitro. Eliminating these cells during the regenerative response that follows glycerol-induced acute tubular necrosis worsened peak renal injury in vivo. Thus, higher clone-forming and developmental capacities characterize a distinct subset of adult kidney-derived cells. The ability to influence an endogenous regenerative response via NCAM1 targeting may lead to novel therapeutics for renal diseases.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco / Antígeno CD56 / Células Epiteliais / Rim Limite: Adult / Animals / Humans Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco / Antígeno CD56 / Células Epiteliais / Rim Limite: Adult / Animals / Humans Idioma: En Ano de publicação: 2013 Tipo de documento: Article