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A chemically synthesized capture agent enables the selective, sensitive, and robust electrochemical detection of anthrax protective antigen.
Farrow, Blake; Hong, Sung A; Romero, Errika C; Lai, Bert; Coppock, Matthew B; Deyle, Kaycie M; Finch, Amethist S; Stratis-Cullum, Dimitra N; Agnew, Heather D; Yang, Sung; Heath, James R.
Afiliação
  • Farrow B; Department of Applied Physics and Materials Science, and ‡Division of Chemistry and Chemical Engineering, California Institute of Technology , 1200 East California Boulevard, Pasadena, California 91125, United States.
ACS Nano ; 7(10): 9452-60, 2013 Oct 22.
Article em En | MEDLINE | ID: mdl-24063758
We report on a robust and sensitive approach for detecting protective antigen (PA) exotoxin from Bacillus anthracis in complex media. A peptide-based capture agent against PA was developed by improving a bacteria display-developed peptide into a highly selective biligand through in situ click screening against a large, chemically synthesized peptide library. This biligand was coupled with an electrochemical enzyme-linked immunosorbent assay utilizing nanostructured gold electrodes. The resultant assay yielded a limit of detection of PA of 170 pg/mL (2.1 pM) in buffer, with minimal sensitivity reduction in 1% serum. The powdered capture agent could be stably stored for several days at 65 °C, and the full electrochemical biosensor showed no loss of performance after extended storage at 40 °C. The engineered stability and specificity of this assay should be extendable to other cases in which biomolecular detection in demanding environments is required.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Toxinas Bacterianas / Técnicas Eletroquímicas / Antígenos de Bactérias Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Toxinas Bacterianas / Técnicas Eletroquímicas / Antígenos de Bactérias Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2013 Tipo de documento: Article