Chromatin immunoprecipitation and deep sequencing in Xenopus tropicalis and Xenopus laevis.
Methods
; 66(3): 410-21, 2014 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-24064036
ABSTRACT
Chromatin immunoprecipitation and deep sequencing (ChIP-SEQ) represents a powerful tool for identifying the genomic targets of transcription factors, chromatin remodeling factors, and histone modifications. The frogs Xenopus laevis and Xenopus tropicalis have historically been outstanding model systems for embryology and cell biology, with emerging utility as highly accessible embryos for genome-wide studies. Here we focus on the particular strengths and limitations of Xenopus cell biology and genomics as they apply to ChIP-SEQ, and outline a methodology for ChIP-SEQ in both species, providing detailed strategies for sample preparation, antibody selection, quality control, sequencing library preparation, and basic analysis.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Xenopus
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Imunoprecipitação da Cromatina
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Sequenciamento de Nucleotídeos em Larga Escala
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article