Enzymatic transformation of nitro-aromatic compounds by a flavin-free NADH azoreductase from Lysinibacillus sphaericus.
Biotechnol Lett
; 36(1): 127-31, 2014 Jan.
Article
em En
| MEDLINE
| ID: mdl-24068503
ABSTRACT
Azo dyes and nitro-aromatic compounds are the largest group of pollutants released in the environment as industrial wastes. They create serious health and environmental problems. Azoreductases catalyze the reduction of azo dyes and nitro compounds to their respective amines. AN azoreductase was purified up to 12-fold from Lysinibacillus sphaericus using ion-exchange and size exclusion chromatography. It was optimally active at pH 7.4 and 75 °C. It was stable at 70 °C for 30 min. The purified enzyme utilized NADH rather than NADPH as an electron donor to reduce substrates. The molecular weight of the purified enzyme was ~29 kDa. The enzyme also acted as nitroreductase and could selectively reduce the nitro group of 2-nitrophenol, 4-nitrobenzoic acid, 2-nitro-benzaldehyde and 3-nitrophenol. Reduction products of these compounds were identified by IR and NMR.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Compostos Azo
/
Bacillaceae
/
NADH NADPH Oxirredutases
/
Nitrocompostos
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article