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Selection of Pseudomonas aeruginosa reference genes for RT-qPCR analysis from sputum of cystic fibrosis patients.
Costaglioli, Patricia; Barthe, Christophe; Fayon, Michael; Christoflour, Naik; Bui, Stéphanie; Derlich, Laurence; Domblides, Philippe; Crouzet, Marc; Vilain, Sébastien; Garbay, Bertrand.
Afiliação
  • Costaglioli P; Univ. Bordeaux, Biotechnologie des Protéines Recombinantes à Visée Santé, EA 4135, F-33000 Bordeaux, France. Electronic address: patricia.costaglioli@ipb.fr.
Mol Cell Probes ; 28(1): 10-2, 2014 Feb.
Article em En | MEDLINE | ID: mdl-24075879
ABSTRACT
The prerequisite to monitor gene expression is the selection of reference genes for normalization of RT-qPCR results. Using 13 sputum samples collected from 9 CF patients, we demonstrated that PA2875 and PA3340 are better reference genes than the previously used clpX and oprL genes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Escarro / Fibrose Cística / Genes Bacterianos Limite: Humans Idioma: En Ano de publicação: 2014 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Escarro / Fibrose Cística / Genes Bacterianos Limite: Humans Idioma: En Ano de publicação: 2014 Tipo de documento: Article