Sample preparation for single molecule localization microscopy.
Phys Chem Chem Phys
; 15(43): 18771-83, 2013 Nov 21.
Article
em En
| MEDLINE
| ID: mdl-24084850
ABSTRACT
Single molecule localization-based optical nanoscopy was introduced in 2006, surpassing traditional diffraction-limited resolutions by an order of magnitude. Seven years later, this superresolution technique is continuing to follow a trend of increasing popularity and pervasiveness, with the proof-of-concept work long finished and commercial implementations now available. However one important aspect that tends to become lost in translation is the importance of proper sample preparation, with very few resources addressing the considerations that must be made when preparing samples for imaging with single molecule level sensitivity. Presented here is a an in-depth analysis of all aspects of sample preparation for single molecule superresolution, including both live and fixed cell preparation, choice of fluorophore, fixation and staining techniques, and imaging buffer considerations.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Complexos de Coordenação
/
Microscopia de Fluorescência
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article