Magnetic resonance imaging of metastases in xenograft mouse models of cancer.
Methods Mol Biol
; 1070: 213-22, 2014.
Article
em En
| MEDLINE
| ID: mdl-24092443
ABSTRACT
Magnetic resonance imaging (MRI) of small animals has emerged as a valuable tool to noninvasively monitor tumor growth in mouse models of cancer. However, imaging of metastases in mouse models is difficult due to the need for high spatial resolution. We have demonstrated MRI of metastases in the liver, brain, adrenal glands, and lymph nodes in different xenograft mouse models of cancer. MRI of mice was performed with a clinical 3.0 T magnetic resonance scanner and a commercially available small-animal receiver coil. The imaging protocol consisted of T1- and T2-weighted fat-saturated spin echo sequences with a spatial resolution of 200 µm × 200 µm × 500 µm. Total acquisition time was 30 min per mouse. The technique allowed for repetitive examinations of larger animal cohorts to observe the development of metastases.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imageamento por Ressonância Magnética
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Ensaios Antitumorais Modelo de Xenoenxerto
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Modelos Animais de Doenças
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Metástase Neoplásica
Tipo de estudo:
Diagnostic_studies
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Guideline
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Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article