Aligning cells in arbitrary directions on a membrane sheet using locally formed microwrinkles.
Biotechnol Lett
; 36(2): 391-6, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-24101251
ABSTRACT
Sheets of cells can be used for tissue regenerative medicine. Cell alignment within the sheet is now a key factor in the next generation of this technology. Anisotropic cell sheets without random cell orientations have been conventionally produced with photolithographically, microfabricated substrates using special facilities and equipment. Here we demonstrate a more accessible approach to the fabrication of anisotropic substrates. We locally deformed part of an elastic membrane and simultaneously oxidized the surface to create microwrinkles as well as to enable adhesion to the extracellular matrix. The approach with the local loading made it possible to orient cells in controlled directions within a single membrane sheet depending on the strains determined by the controllable deformation. This technique potentially enables a versatile design of microwrinkles for target-compatible cell alignments.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Engenharia Tecidual
/
Medicina Regenerativa
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Alicerces Teciduais
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Membranas
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article