Cell culture condition-dependent impact of AGE-rich food extracts on kinase activation and cell survival on human fibroblasts.
Int J Food Sci Nutr
; 65(2): 219-25, 2014 Mar.
Article
em En
| MEDLINE
| ID: mdl-24111510
ABSTRACT
Advanced glycation end products (AGEs) are stable end products of the Maillard reaction. Effects of food extracts are often initially analysed in cellular test systems and it is not clear how different cell culture conditions might influence the results. Therefore, we compared the effects of two models for AGE-rich food, bread crust and coffee extract (CE) on WI-38 human lung fibroblasts under different cell culture conditions (sub-confluent versus confluent cells, with and without serum). WI-38 cells responded to coffee and bread crust extract (BCE) with a rapid phosphorylation of PKB (AKT), p42/44 MAPK (ERK 1/2) and p38 MAPK, strongly depending on culture conditions. BCE resulted in increased cell numbers, whereas CE appeared to be cytotoxic. When cell numbers under all culture conditions and treatments were correlated with kinase phosphorylation, the relation between phospho-p38 MAPK and phospho-AKT represented a good, cell culture condition-independent predictor of cell survival.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pão
/
Sobrevivência Celular
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Produtos Finais de Glicação Avançada
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Café
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Técnicas de Cultura de Células
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Proteínas Quinases Ativadas por Mitógeno
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Fibroblastos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article