Novel Aeruginosin-865 from Nostoc sp. as a potent anti-inflammatory agent.
Chembiochem
; 14(17): 2329-37, 2013 Nov 25.
Article
em En
| MEDLINE
| ID: mdl-24123716
ABSTRACT
Aeruginosin-865 (Aer-865), isolated from terrestrial cyanobacterium Nostoc sp. Lukesová 30/93, is the first aeruginosin-type peptide containing both a fatty acid and a carbohydrate moiety, and is the first aeruginosin to be found in the genus Nostoc. Mass spectrometry, chemical and spectroscopic analysis as well as one- and two-dimensional NMR and chiral HPLC analysis of Marfey derivatives were applied to determine the peptidic sequence D-Hpla, D-Leu, 5-OH-Choi, Agma, with hexanoic and mannopyranosyl uronic acid moieties linked to Choi. We used an AlphaLISA assay to measure the levels of proinflammatory mediators IL-8 and ICAM-1 in hTNF-α-stimulated HLMVECs. Aer-865 showed significant reduction of both with EC50 values of (3.5±1.5) µg mL(-1) ((4.0±1.7) µM) and (50.0±13.4) µg mL(-1) ((57.8±15.5) µM), respectively. Confocal laser scanning microscopy revealed that the anti-inflammatory effect of Aer-865 was directly associated with inhibition of NF-κB translocation to the nucleus. Moreover, Aer-865 did not show any cytotoxic effect.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Glicopeptídeos
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Anti-Inflamatórios não Esteroides
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Nostoc
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Lipopeptídeos
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article