ATR/Mec1 prevents lethal meiotic recombination initiation on partially replicated chromosomes in budding yeast.
Elife
; 2: e00844, 2013 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-24137535
ABSTRACT
During gamete formation, crossover recombination must occur on replicated DNA to ensure proper chromosome segregation in the first meiotic division. We identified a Mec1/ATR- and Dbf4-dependent replication checkpoint in budding yeast that prevents the earliest stage of recombination, the programmed induction of DNA double-strand breaks (DSBs), when pre-meiotic DNA replication was delayed. The checkpoint acts through three complementary mechanisms inhibition of Mer2 phosphorylation by Dbf4-dependent Cdc7 kinase, preclusion of chromosomal loading of Rec114 and Mre11, and lowered abundance of the Spo11 nuclease. Without this checkpoint, cells formed DSBs on partially replicated chromosomes. Importantly, such DSBs frequently failed to be repaired and impeded further DNA synthesis, leading to a rapid loss in cell viability. We conclude that a checkpoint-dependent constraint of DSB formation to duplicated DNA is critical not only for meiotic chromosome assortment, but also to protect genome integrity during gametogenesis. DOIhttp//dx.doi.org/10.7554/eLife.00844.001.
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Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
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Saccharomyces cerevisiae
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Cromossomos Fúngicos
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Proteínas Serina-Treonina Quinases
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Proteínas de Saccharomyces cerevisiae
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Peptídeos e Proteínas de Sinalização Intracelular
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Replicação do DNA
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Meiose
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article