[Anti-human 4-1BBL monoclonal antibody stimulates the nuclear translocation of NF-κB and the co-location of 4-1BBL/CD28 isoform in U937 cells].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 21(5): 1121-5, 2013 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-24156418
ABSTRACT
This study was purposed to investigate the molecular mechanism of 4-1BBL reverse signals in the human acute monocytic leukemia cell line of U937. The U937 cell line was used as target cells, and stimulated by the mouse anti-human 4-1BBL monoclonal antibody 1F1. The nuclear translocation of NF-κB and the co-location of 4-1BBL and CD28i molecules in U937 cells were observed with confocal laser scanning microscopy. The protein and m-RNA expression levels of 4-1BBL and CD28i were detected by flow cytometry and RT-PCR respectively. The results showed that the significant nuclear translocation of NF-κB and co-localization of 4-1BBL and CD28i on membrane of U937 cells appeared after being stimulated by mAb1F1. It is concluded that the 4-1BBL reverse signals transduction mediating the growth of U937 cells relates with the nuclear translocation of NF-κB. CD28i may be involved in intracellular 4-1BBL reverse signaling pathways.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
NF-kappa B
/
Antígenos CD28
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Ligante 4-1BB
/
Anticorpos Monoclonais
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2013
Tipo de documento:
Article