Targeted gene deletion in Aspergillus fumigatus using microbial machinery and a recyclable marker.
J Microbiol Methods
; 95(3): 373-8, 2013 Dec.
Article
em En
| MEDLINE
| ID: mdl-24161898
ABSTRACT
The emerging invasive fungal pathogen Aspergillus fumigatus causes very serious infections among immunocompromised patient populations. While the genome of this pathogen has been sequenced, a major barrier to better understanding the complex biology of this eukaryotic organism is a lack of tools for efficient genetic manipulation. To improve upon this, we have generated a new gene deletion system for A. fumigatus using yeast recombinational cloning and Agrobacterium tumefaciens mediated transformation (ATMT) employing a recyclable marker system. This system reduced the time for generating a gene deletion strain in our hands by two-thirds (12 weeks to 3 weeks) using minimal human labor, and we demonstrate that it can be used to efficiently generate multiple gene deletions within a single strain.
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Base de dados:
MEDLINE
Assunto principal:
Aspergillus fumigatus
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Marcadores Genéticos
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Técnicas de Inativação de Genes
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Genética Microbiana
Tipo de estudo:
Evaluation_studies
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article