An improve method for somatic embryogenesis of Schisandra chinensis (Turcz.) Baillon.
Pak J Biol Sci
; 16(3): 127-34, 2013 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-24171274
ABSTRACT
A somatic embryogenesis protocol for plant regeneration of Schisandra chinensis (Turcz.) Baillon was established. Increased callus induction was obtained from mature zygotic embryos on Murashige and Skoog (MS) medium supplemented with 1.0 mg L(-1) N-phenyl-1,2,3-thidiazol-5-yl-urea (TDZ) or 2.0 mg L(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). Addition of Zeatin (Zt) promoted the formation of embryogenic calli. To induce somatic embryogenesis, 2,4-D, TDZ and Zt were incorporated in the medium alone or in combination. Development of the maximum number of somatic embryos (81 globular, 37 heart, 52 torpedo and 37 cotyledon-stage) and germination of the highest number of embryos (50%) was observed on 1/2 MS medium supplemented with 1.0 mg L(-1) TDZ and 0.2 mg L(-1) Zt. Further development of somatic embryos into plantlets was completed in 1/2 MS medium free of plant growth regulators.
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Base de dados:
MEDLINE
Assunto principal:
Schisandra
/
Técnicas de Embriogênese Somática de Plantas
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article