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An in vitro method to investigate the microbicidal potential of human macrophages for use in psychosomatic research.
Kuebler, Ulrike; Ehlert, Ulrike; Zuccarella, Claudia; Sakai, Miho; Stemmer, Andreas; Wirtz, Petra H.
Afiliação
  • Kuebler U; Department of Psychology, Biological and Health Psychology, University of Bern, Alpeneggstrasse 22, 3012 Bern, Switzerland. petra.wirtz@psy.unibe.ch.
Psychosom Med ; 75(9): 841-8, 2013.
Article em En | MEDLINE | ID: mdl-24184844
ABSTRACT

OBJECTIVE:

Psychological states relate to changes in circulating immune cells, but associations with immune cells in peripheral tissues such as macrophages have hardly been investigated. Here, we aimed to implement and validate a method for measuring the microbicidal potential of ex vivo isolated human monocyte-derived macrophages (HMDMs) as an indicator of macrophage activation.

METHODS:

The method was implemented and validated for two blood sampling procedures (short-term cannula insertion versus long-term catheter insertion) in 79 participants (34 women, 45 men) aged between 18 and 75 years. The method principle is based on the reduction of 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-dis-ulfophenyl)-2H-tetrazolium, monosodium salt (WST-1) by superoxide anions, the first in a series of pathogen-killing reactive oxygen species produced by phorbol myristate acetate-activated HMDM. Cytochrome c reduction and current generation were measured as reference methods for validation purposes. We further evaluated whether depressive symptom severity (Beck Depression Inventory) and chronic stress (Chronic Stress Screening Scale) were associated with macrophage microbicidal potential.

RESULTS:

The assay induced superoxide anion responses by HMDM in all participants. Assay results depended on blood sampling procedure (cannula versus catheter insertion). Interassay variability as a measure for assay reliability was 10.92% or less. WST-1 reduction scores correlated strongly with results obtained by reference methods (cytochrome c r = 0.57, p = .026; current generation r values ≥ 0.47, p values <.033) and with psychological factors (depressive symptom severity r = 0.35 [cannula insertion] versus r = -0.54 [catheter insertion]; chronic stress r = 0.36 [cannula insertion]; p values ≤ .047).

CONCLUSIONS:

Our findings suggest that the implemented in vitro method investigates microbicidal potential of HMDM in a manner that is valid and sensitive to psychological measures.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Medicina Psicossomática / Estresse Psicológico / Sais de Tetrazólio / Depressão / Macrófagos Limite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Medicina Psicossomática / Estresse Psicológico / Sais de Tetrazólio / Depressão / Macrófagos Limite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2013 Tipo de documento: Article