Molecular and bioenergetic differences between cells with African versus European inherited mitochondrial DNA haplogroups: implications for population susceptibility to diseases.
Biochim Biophys Acta
; 1842(2): 208-19, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-24200652
ABSTRACT
The geographic origins of populations can be identified by their maternally inherited mitochondrial DNA (mtDNA) haplogroups. This study compared human cybrids (cytoplasmic hybrids), which are cell lines with identical nuclei but mitochondria from different individuals with mtDNA from either the H haplogroup or L haplogroup backgrounds. The most common European haplogroup is H while individuals of maternal African origin are of the L haplogroup. Despite lower mtDNA copy numbers, L cybrids had higher expression levels for nine mtDNA-encoded respiratory complex genes, decreased ATP (adenosine triphosphate) turnover rates and lower levels of reactive oxygen species production, parameters which are consistent with more efficient oxidative phosphorylation. Surprisingly, GeneChip arrays showed that the L and H cybrids had major differences in expression of genes of the canonical complement system (5 genes), dermatan/chondroitin sulfate biosynthesis (5 genes) and CCR3 (chemokine, CC motif, receptor 3) signaling (9 genes). Quantitative nuclear gene expression studies confirmed that L cybrids had (a) lower expression levels of complement pathway and innate immunity genes and (b) increased levels of inflammation-related signaling genes, which are critical in human diseases. Our data support the hypothesis that mtDNA haplogroups representing populations from different geographic origins may play a role in differential susceptibilities to diseases.
Palavras-chave
ABI; ARPE-19; ATP; Adenosine triphosphate; Applied Biosystems; C1s; C3; C4B; CFH; Carbonyl Cyanide 4-trifluoromethoxy-phenylhydrazone; Complement activation; Complement component 1, s subcomponent; Complement component 3; Complement component 4B; Complement factor H; Cybrid; DMEM; DNA; Deoxyribonucleic acid; Dulbecco's modified Eagle's medium; ECAR; EDTA; ETC; Electron transport chain; Ethylenediaminetetracetic acid; Extracellular acidification rate; FCCP; Haplogroup; Innate immunity; MT-CO1; MT-CO2; MT-CO3; MT-CYB; MT-ND1; MT-ND3; MT-ND5; Maximal oxygen uptake; MicroMolar; Mitochondria encoded NADH dehydrogenase 1; Mitochondria encoded NADH dehydrogenase 3; Mitochondria encoded NADH dehydrogenase 5; Mitochondria encoded cytochrome B; Mitochondria encoded cytochrome oxidase 1; Mitochondria encoded cytochrome oxidase 2; Mitochondria encoded cytochrome oxidase 3; Mitochondrion; OCR; OXPHOS; Oxidative phosphorylation; Oxygen consumption rate; Q-PCR; Quantitative polymerase chain reaction; Retina; Retinal pigmented epithelium cell line; SEM; SNPs; Single nucleotide polymorphisms; Standard error mean; UCLA; University of California, Los Angeles; VO2(max); µM
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Haplótipos
/
DNA Mitocondrial
/
População Negra
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População Branca
/
Metabolismo Energético
Tipo de estudo:
Prognostic_studies
Limite:
Adult
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Humans
/
Middle aged
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article