Second site reversion of a mutation near the amino terminus of the HIV-1 capsid protein.
Virology
; 447(1-2): 95-103, 2013 Dec.
Article
em En
| MEDLINE
| ID: mdl-24210103
ABSTRACT
During HIV-1 morphogenesis, the precursor Gag protein is processed to release capsid (CA) proteins that form the mature virus core. In this process, the CA proteins assemble a lattice in which N-terminal domain (NTD) helices 1-3 are critical for multimer formation. Mature core assembly requires refolding of the N-terminus of CA into a ß-hairpin, but the precise contribution of the hairpin core morphogenesis is unclear. We found that mutations at isoleucine 15 (I15), between the ß-hairpin and NTD helix 1 are incompatible with proper mature core assembly. However, a compensatory mutation of histidine 12 in the ß-hairpin to a tyrosine was selected by long term passage of an I15 mutant virus in T cells. The tyrosine does not interact directly with residue 15, but with NTD helix 3, supporting a model in which ß-hairpin folding serves to align helix 3 for mature NTD multimerization.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Supressão Genética
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HIV-1
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Proteína do Núcleo p24 do HIV
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Mutação de Sentido Incorreto
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article