[Pre-freezing equilibration improves post-thaw human semen parameters].
Zhonghua Nan Ke Xue
; 19(10): 886-9, 2013 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-24218940
ABSTRACT
OBJECTIVE:
To investigate the effect of pre-freezing equilibration on the cryo-survival of human sperm and to optimize the protocol of direct fumigation for the freeze-thawing of human sperm.METHODS:
We collected 50 semen samples from healthy donors, each subjected to cryopreservation with 3 differentmethods:
non-equilibration freezing (Group A), 10-min equilibration at room temperature before freezing (Group B), and 10-min equilibration at 4 degrees C before freezing (Group C). We examined all the post-thaw semen samples by computer-assisted semen analysis for the sperm motility parameters, and detected the sperm vitality and deformity index (SDI).RESULTS:
The recovery rate of progressive sperm motility was (61.88 +/- 16.94)% in Group C, remarkably higher than in A ([48.61 +/- 16.44]%) and B ([49.41 +/- 13.77]%) (P < 0.05), but with no significant difference between the latter two. And there were no significant differences in sperm vitality and SDI among the three groups.CONCLUSION:
Ten-minute equilibration at 4 degrees C before freezing can evidently improve the progressive motility of sperm in addition to its advantages of easy operation and controllable experimental condition.
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Base de dados:
MEDLINE
Assunto principal:
Preservação do Sêmen
/
Criopreservação
/
Análise do Sêmen
Limite:
Adult
/
Humans
/
Male
Idioma:
Zh
Ano de publicação:
2013
Tipo de documento:
Article