Essential yet limited role for CCR2⺠inflammatory monocytes during Mycobacterium tuberculosis-specific T cell priming.
Elife
; 2: e01086, 2013 Nov 12.
Article
em En
| MEDLINE
| ID: mdl-24220507
Defense against infection by Mycobacterium tuberculosis (Mtb) is mediated by CD4 T cells. CCR2(+) inflammatory monocytes (IMs) have been implicated in Mtb-specific CD4 T cell responses but their in vivo contribution remains unresolved. Herein, we show that transient ablation of IMs during infection prevents Mtb delivery to pulmonary lymph nodes, reducing CD4 T cell responses. Transfer of MHC class II-expressing IMs to MHC class II-deficient, monocyte-depleted recipients, while restoring Mtb transport to mLNs, does not enable Mtb-specific CD4 T cell priming. On the other hand, transfer of MHC class II-deficient IMs corrects CD4 T cell priming in monocyte-depleted, MHC class II-expressing mice. Specific depletion of classical DCs does not reduce Mtb delivery to pulmonary lymph nodes but markedly reduces CD4 T cell priming. Thus, although IMs acquire characteristics of DCs while delivering Mtb to lymph nodes, cDCs but not moDCs induce proliferation of Mtb-specific CD4 T cells. DOI: http://dx.doi.org/10.7554/eLife.01086.001.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Monócitos
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Linfócitos T
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Receptores CCR2
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Inflamação
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Mycobacterium tuberculosis
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article