Expression of VEGF-A, Otx homeobox and p53 family genes in proliferative vitreoretinopathy.
Mediators Inflamm
; 2013: 857380, 2013.
Article
em En
| MEDLINE
| ID: mdl-24227910
ABSTRACT
INTRODUCTION:
Proliferative vitreoretinopathy (PVR) is a severe inflammatory complication of retinal detachment. Pathological epiretinal membranes grow on the retina surface leading to contraction, and surgery fails in 5% to 10% of the cases. We evaluated the expression of VEGF-A, Otx1, Otx2, Otx3, and p53 family members from PVR specimens to correlate their role in inducing or preventing the pathology.METHODS:
Twelve retinal samples were taken from patients affected by PVR during therapeutic retinectomies in vitreoretinal surgery. Gene expression was evaluated using quantitative real-time reverse transcriptase PCR analysis and immunohistochemistry, using four healthy human retinae as control.RESULT:
Controls showed basal expression of all genes. PVR samples showed little or no expression of Otx1 and variable expression of VEGF-A, Otx2, Otx3, p53, and p63 genes. Significant correlation was found among VEGF-A, Otx2, p53, and p63 and between Otx1 and Otx3.CONCLUSIONS:
Otx homeobox, p53 family, and VEGF-A genes are expressed in PVR human retina. We individuated two possible pathways (VEGF-A, Otx2, p53, p63 and Otx1 and Otx3) involved in PVR progression that could influence in different manners the course of the pathology. Individuating the genetic pathways of PVR represents a novel approach to PVR therapies.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Retina
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Proteína Supressora de Tumor p53
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Vitreorretinopatia Proliferativa
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Fator A de Crescimento do Endotélio Vascular
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Fatores de Transcrição Otx
Limite:
Aged
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Aged80
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article