The relation between body iron stores and adipose tissue function in patients with manifest vascular disease.
Eur J Clin Invest
; 43(12): 1240-9, 2013 Dec.
Article
em En
| MEDLINE
| ID: mdl-24245570
ABSTRACT
BACKGROUND:
We investigated whether plasma ferritin levels through the pro-inflammatory effects of free iron are associated with adipose tissue dysfunction in a relevant population of patients with manifest vascular disease who would potentially benefit the most from further aetiological insights. MATERIALS ANDMETHODS:
In a cohort of 355 patients with vascular diseases, the association between plasma ferritin and adiponectin levels was quantified using linear regression analysis. Interleukin-6 and adiponectin levels were measured in medium from pre-adipocytes and adipocytes after incubation with increasing concentrations of Fe(III)-citrate and after co-incubation with iron chelators or radical scavengers.RESULTS:
Increasing ferritin plasma concentrations were not related to plasma adiponectin levels in patients without (ß -0·13; 95% CI -0·30 to 0·04) or with the metabolic syndrome (ß -0·04; 95% CI -0·17 to 0·10). Similar results were found in patients who developed a new cardiovascular event in the follow-up period. In vitro, incubation with increasing concentrations of Fe(III)-citrate-induced inflammation in pre-adipocyte cultures as witnessed by increased IL-6 secretion at 30 µm Fe(III)-citrate vs. control (500 ± 98 pg/mL vs. 194 ± 31 pg/mL, P = 0·03). Co-incubation of pre-adipocytes with iron chelators or radical scavengers prevented this inflammatory response. Incubation of adipocytes with 30 µm Fe(III)-citrate did not influence adiponectin secretion compared with control.CONCLUSIONS:
In patients with vascular disease, there is no association between plasma ferritin and adiponectin levels. In vitro, free iron induces an inflammatory response in pre-adipocytes, but not in adipocytes. This response was blocked by co-incubation with iron chelators or radical scavengers. Adiponectin secretion by adipocytes was not influenced by free iron.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tecido Adiposo
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Aterosclerose
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Ferritinas
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Female
/
Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article