Characterization and incompatibility properties of ROM- derivatives of pBR322-based plasmids.
J Gen Microbiol
; 132(4): 1021-6, 1986 Apr.
Article
em En
| MEDLINE
| ID: mdl-2428924
ABSTRACT
We have characterized a copy number mutant of the pBR322-based plasmid pWT111. A single nucleotide transversion in loop II' of RNAI results in an eightfold increase in plasmid copy number. Removal of the rom coding region from pWT111 cop results in a further sixfold increase in copy number. We present evidence that ROM is involved in the strong incompatibility effect seen between pMB1 and ColE1 type plasmids.
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Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Escherichia coli
Idioma:
En
Ano de publicação:
1986
Tipo de documento:
Article