Antigenic analysis of monoclonal antibodies against different epitopes of σB protein of avian reovirus.
PLoS One
; 8(11): e81533, 2013.
Article
em En
| MEDLINE
| ID: mdl-24312314
ABSTRACT
BACKGROUND:
Avian reovirus (ARV) causes arthritis, tenosynovitis, runting-stunting syndrome (RSS), malabsorption syndrome (MAS) and immunosuppression in chickens. σB is one of the major structural proteins of ARV, which is able to induce group-specific antibodies against the virus. METHODS ANDRESULTS:
The present study described the identification of two linear B-cell epitopes in ARV σB through expressing a set of partially overlapping and consecutive truncated peptides spanning σB screened with two monoclonal antibodies (mAbs) 1F4 and 1H3-1.The data indicated that (21)KTPACW(26) (epitope A) and (32)WDTVTFH(38) (epitope B) were minimal determinants of the linear B cell epitopes. Antibodies present in the serum of ARV-positive chickens recognized the minimal linear epitopes in Western blot analyses. By sequence alignment analysis, we determined that the epitopes A and B were not conserved among ARV, duck reovirus (DRV) and turkey reovirus (TRV) strains. Western blot assays, confirmed that epitopes A and B were ARV-specific epitopes, and they could not react with the corresponding peptides of DRV and TRV. CONCLUSIONS ANDSIGNIFICANCE:
We identified (21)KTPACW(26) and (32)WDTVTFH(38) as σB -specific epitopes recognized by mAbs 1F4 and 1H3-1, respectively. The results in this study may have potential applications in development of diagnostic techniques and epitope-based marker vaccines against ARV groups.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Epitopos de Linfócito B
/
Orthoreovirus Aviário
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Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article