Effects of aging, cytomegalovirus infection, and EBV infection on human B cell repertoires.
J Immunol
; 192(2): 603-11, 2014 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-24337376
ABSTRACT
Elderly humans show decreased humoral immunity to pathogens and vaccines, yet the effects of aging on B cells are not fully known. Chronic viral infection by CMV is implicated as a driver of clonal T cell proliferations in some aging humans, but whether CMV or EBV infection contributes to alterations in the B cell repertoire with age is unclear. We have used high-throughput DNA sequencing of IGH gene rearrangements to study the BCR repertoires over two successive years in 27 individuals ranging in age from 20 to 89 y. Some features of the B cell repertoire remain stable with age, but elderly subjects show increased numbers of B cells with long CDR3 regions, a trend toward accumulation of more highly mutated IgM and IgG Ig genes, and persistent clonal B cell populations in the blood. Seropositivity for CMV or EBV infection alters B cell repertoires, regardless of the individual's age EBV infection correlates with the presence of persistent clonal B cell expansions, whereas CMV infection correlates with the proportion of highly mutated Ab genes. These findings isolate effects of aging from those of chronic viral infection on B cell repertoires and provide a baseline for understanding human B cell responses to vaccination or infectious stimuli.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Envelhecimento
/
Linfócitos B
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Infecções por Citomegalovirus
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Herpesvirus Humano 4
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Infecções por Vírus Epstein-Barr
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Citomegalovirus
Limite:
Adult
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Aged
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Aged80
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Humans
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Middle aged
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article