Analysis of superoxide dismutase 1, dual-specificity phosphatase 1, and transforming growth factor, beta 1 genes expression in keratoconic and non-keratoconic corneas.
Mol Vis
; 19: 2501-7, 2013.
Article
em En
| MEDLINE
| ID: mdl-24339725
ABSTRACT
PURPOSE:
To quantitatively assess the superoxide dismutase 1 (SOD1), transforming growth factor, beta 1 (TGF-ß1), and dual-specificity phosphatase 1 (DUSP1) messenger ribonucleic acid (mRNA) expression levels as the main intracellular reactive oxygen species neutralizers, wound healing mediators, and immunomodulators (respectively) in keratoconic (KCN) and non-KCN corneas.METHODS:
Total RNA was extracted from normal and keratoconic cultured corneal stromal fibroblasts. Semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to measure the relative expression levels of mRNAs of the SOD1, TGF-ß1, and DUSP1 genes.RESULTS:
The mRNA expression of TGF-ß1 and DUSP1 was augmented in the KCN corneas (three- and fivefold, respectively; both p<0.05). The KCN and non-KCN samples showed no difference in comparative SOD1 mRNA levels.CONCLUSIONS:
This study demonstrated a higher level of DUSP1 and TGF-ß1 expression as known molecules in the inflammatory process. These results may provide new insight into the complex molecular pathways underlying KCN for investigating other inflammatory molecules.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
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Fator de Crescimento Transformador beta1
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Fosfatase 1 de Especificidade Dupla
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Fibroblastos
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Ceratocone
Limite:
Female
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Humans
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Male
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article