House dust exposure mediates gut microbiome Lactobacillus enrichment and airway immune defense against allergens and virus infection.
Proc Natl Acad Sci U S A
; 111(2): 805-10, 2014 Jan 14.
Article
em En
| MEDLINE
| ID: mdl-24344318
ABSTRACT
Exposure to dogs in early infancy has been shown to reduce the risk of childhood allergic disease development, and dog ownership is associated with a distinct house dust microbial exposure. Here, we demonstrate, using murine models, that exposure of mice to dog-associated house dust protects against ovalbumin or cockroach allergen-mediated airway pathology. Protected animals exhibited significant reduction in the total number of airway T cells, down-regulation of Th2-related airway responses, as well as mucin secretion. Following dog-associated dust exposure, the cecal microbiome of protected animals was extensively restructured with significant enrichment of, amongst others, Lactobacillus johnsonii. Supplementation of wild-type animals with L. johnsonii protected them against both airway allergen challenge or infection with respiratory syncytial virus. L. johnsonii-mediated protection was associated with significant reductions in the total number and proportion of activated CD11c(+)/CD11b(+) and CD11c(+)/CD8(+) cells, as well as significantly reduced airway Th2 cytokine expression. Our results reveal that exposure to dog-associated household dust results in protection against airway allergen challenge and a distinct gastrointestinal microbiome composition. Moreover, the study identifies L. johnsonii as a pivotal species within the gastrointestinal tract capable of influencing adaptive immunity at remote mucosal surfaces in a manner that is protective against a variety of respiratory insults.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Hiper-Reatividade Brônquica
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Infecções por Vírus Respiratório Sincicial
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Trato Gastrointestinal
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Poeira
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Exposição Ambiental
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Microbiota
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Lactobacillus
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article