The prevalence of sperm with large nuclear vacuoles is a prognostic tool in the prediction of ICSI success.

ABSTRACT

PURPOSE: To investigate if there is a correlation between the prevalence of sperm with large nuclear vacuoles (LNV) and intracytoplasmic sperm injection (ICSI) outcomes. METHODS: Two hundred male patients undergoing ICSI had their sperm morphology evaluated through motile sperm organelle morphology examination (MSOME) and the percentage of LNV sperm was recorded and correlated to the ICSI outcomes. RESULTS: The percentage of sperm with LNV negatively influenced the blastocyst formation (S 16.9, R(2) 20.5%, p = 0.004) and implantation (S 34.7, R(2) 26.2%, p = 0.001). There were significant differences in the percentage of sperm with LNV between patients in which pregnancy was achieved or not (22.2% vs. 28.4%, p < 0.001) and in patients with ongoing pregnancy or not (22.4% vs. 28.5%, p < 0.001). The incidence of sperm with LNV was determinant to the decreased odds of pregnancy (OR 0.74, p < 0.001) and increased odds of miscarriage (OR 1.46, p < 0.001). The area under the curve (AUC) was sufficient to distinguish between couples which did achieve pregnancy or not (AUC 0.922, p < 0.001). CONCLUSIONS: The MSOME is a prognostic tool in the prediction of ICSI success and could be used to select patients that should have their sperm selected by MSOME for ICSI.