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Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography.
Johansson, Linda C; Arnlund, David; Katona, Gergely; White, Thomas A; Barty, Anton; DePonte, Daniel P; Shoeman, Robert L; Wickstrand, Cecilia; Sharma, Amit; Williams, Garth J; Aquila, Andrew; Bogan, Michael J; Caleman, Carl; Davidsson, Jan; Doak, R Bruce; Frank, Matthias; Fromme, Raimund; Galli, Lorenzo; Grotjohann, Ingo; Hunter, Mark S; Kassemeyer, Stephan; Kirian, Richard A; Kupitz, Christopher; Liang, Mengning; Lomb, Lukas; Malmerberg, Erik; Martin, Andrew V; Messerschmidt, Marc; Nass, Karol; Redecke, Lars; Seibert, M Marvin; Sjöhamn, Jennie; Steinbrener, Jan; Stellato, Francesco; Wang, Dingjie; Wahlgren, Weixaio Y; Weierstall, Uwe; Westenhoff, Sebastian; Zatsepin, Nadia A; Boutet, Sébastien; Spence, John C H; Schlichting, Ilme; Chapman, Henry N; Fromme, Petra; Neutze, Richard.
Afiliação
  • Johansson LC; Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
  • Arnlund D; Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
  • Katona G; Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
  • White TA; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
  • Barty A; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
  • DePonte DP; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
  • Shoeman RL; 1] Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
  • Wickstrand C; Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
  • Sharma A; Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
  • Williams GJ; Linac Coherent Light Source, LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, 94025 California, USA.
  • Aquila A; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
  • Bogan MJ; PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, 94025 California, USA.
  • Caleman C; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
  • Davidsson J; Department of Chemistry-Ångström Laboratory, Uppsala University, Box 523, 75120 Uppsala, Sweden.
  • Doak RB; Department of Physics, Arizona State University, Tempe, 85287 Arizona, USA.
  • Frank M; Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, 94550 California, USA.
  • Fromme R; Department of Chemistry and Biochemistry, Arizona State University, Tempe, 85287-1604 Arizona, USA.
  • Galli L; 1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] Department of Physics, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.
  • Grotjohann I; Department of Chemistry and Biochemistry, Arizona State University, Tempe, 85287-1604 Arizona, USA.
  • Hunter MS; Department of Chemistry and Biochemistry, Arizona State University, Tempe, 85287-1604 Arizona, USA.
  • Kassemeyer S; 1] Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
  • Kirian RA; Department of Physics, Arizona State University, Tempe, 85287 Arizona, USA.
  • Kupitz C; Department of Chemistry and Biochemistry, Arizona State University, Tempe, 85287-1604 Arizona, USA.
  • Liang M; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
  • Lomb L; 1] Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
  • Malmerberg E; Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
  • Martin AV; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
  • Messerschmidt M; Linac Coherent Light Source, LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, 94025 California, USA.
  • Nass K; 1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] Department of Physics, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.
  • Redecke L; Joint Laboratory for Structural Biology of Infection and Inflammation, Institute of Biochemistry and Molecular Biology, University of Hamburg and Institute of Biochemistry, University of Lübeck at DESY, 22607 Hamburg, Germany.
  • Seibert MM; Linac Coherent Light Source, LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, 94025 California, USA.
  • Sjöhamn J; Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
  • Steinbrener J; 1] Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
  • Stellato F; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
  • Wang D; Department of Physics, Arizona State University, Tempe, 85287 Arizona, USA.
  • Wahlgren WY; Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
  • Weierstall U; Department of Physics, Arizona State University, Tempe, 85287 Arizona, USA.
  • Westenhoff S; Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
  • Zatsepin NA; Department of Physics, Arizona State University, Tempe, 85287 Arizona, USA.
  • Boutet S; Linac Coherent Light Source, LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, 94025 California, USA.
  • Spence JC; Department of Physics, Arizona State University, Tempe, 85287 Arizona, USA.
  • Schlichting I; 1] Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany.
  • Chapman HN; 1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] Department of Physics, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.
  • Fromme P; Department of Chemistry and Biochemistry, Arizona State University, Tempe, 85287-1604 Arizona, USA.
  • Neutze R; Department of Chemistry and Molecular Biology, University of Gothenburg, 405 30 Gothenburg, Sweden.
Nat Commun ; 4: 2911, 2013.
Article em En | MEDLINE | ID: mdl-24352554
ABSTRACT
Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cristalografia por Raios X / Hyphomicrobiaceae / Complexo de Proteínas do Centro de Reação Fotossintética Idioma: En Ano de publicação: 2013 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cristalografia por Raios X / Hyphomicrobiaceae / Complexo de Proteínas do Centro de Reação Fotossintética Idioma: En Ano de publicação: 2013 Tipo de documento: Article