Time-resolved surface-enhanced IR-absorption spectroscopy of direct electron transfer to cytochrome c oxidase from R. sphaeroides.
Biophys J
; 105(12): 2706-13, 2013 Dec 17.
Article
em En
| MEDLINE
| ID: mdl-24359742
ABSTRACT
Time-resolved surface-enhanced IR-absorption spectroscopy triggered by electrochemical modulation has been performed on cytochrome c oxidase from Rhodobacter sphaeroides. Single bands isolated from a broad band in the amide I region using phase-sensitive detection were attributed to different redox centers. Their absorbances changing on the millisecond timescale could be fitted to a model based on protonation-dependent chemical reaction kinetics established previously. Substantial conformational changes of secondary structures coupled to redox transitions were revealed.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
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Rhodobacter sphaeroides
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Complexo IV da Cadeia de Transporte de Elétrons
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Elétrons
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article