Metabolomic characterization of nipple aspirate fluid by (1)H NMR spectroscopy and GC-MS.
J Proteome Res
; 13(2): 883-9, 2014 Feb 07.
Article
em En
| MEDLINE
| ID: mdl-24364541
ABSTRACT
Nipple aspirate fluid (NAF) is a noninvasively obtained biofluid from the duct openings of the breast. NAF components are constantly secreted, metabolized, and reabsorbed by the epithelial lining of the lactiferous ducts of the breast. NAF has been studied as a potential breast tissue surrogate for the discovery of novel breast cancer risk, early detection, and treatment response biomarkers. We report the first unsupervised metabolite characterization of nipple aspirate fluid using NMR and GC-MS using convenience samples previously collected from four premenopausal and four postmenopausal women. A total of 38 metabolites were identified using the two analytical techniques, including amino acids, organic acids, fatty acids, and carbohydrates. Analytical reproducibility of metabolites in NAF by GC-MS was high across different extraction and analysis days. Overall, 31 metabolites had a coefficient of variation below 20%. By GC-MS, there were eight metabolites unique to NAF, 19 unique to plasma, and 24 shared metabolites. Correlative analysis of shared metabolites between matched NAF and plasma samples from pre- and postmenopausal women shows almost no correlations, with the exception being lactic acid, which was significantly negatively correlated (R(2) = 0.57; P = 0.03). These results suggest that NAF is metabolically distinct from plasma and that the application of metabolomic strategies may be useful for future studies investigating breast cancer risk and intervention response biomarkers.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Líquidos Corporais
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Espectroscopia de Ressonância Magnética
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Metabolômica
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Cromatografia Gasosa-Espectrometria de Massas
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Mamilos
Tipo de estudo:
Screening_studies
Limite:
Female
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Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article