Curcumin ameliorated diabetic neuropathy partially by inhibition of NADPH oxidase mediating oxidative stress in the spinal cord.
Neurosci Lett
; 560: 81-5, 2014 Feb 07.
Article
em En
| MEDLINE
| ID: mdl-24370596
ABSTRACT
Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases are the main enzymes that produce oxidative stress, which plays an important role in painful diabetic neuropathy. Curcumin has been reported to exert an antinociceptive effect in a rat model of diabetic neuropathy by suppressing oxidative stress in the spinal cord. However, it remains unknown whether the mechanism by which curcumin ameliorates diabetic neuropathy can be attributed to spinal NADPH oxidases. This study was designed to determine the effect of curcumin on diabetic neuropathy and to investigate its precise mechanism in relation to NADPH oxidase-mediating oxidative stress in the spinal cord. Diabetic neuropathy was induced in Sprague-Dawley rats by intraperitoneal injection with 1% streptozotocin (STZ; 60 mg/kg). After the onset of diabetic neuropathy, a subset of the diabetic rats received daily intragastric administrations of curcumin (200mg/kg) or intraperitoneal injections of apocynin (2.5mg/kg) for 14 consecutive days, whereas other diabetic rats received equivalent volumes of normal saline (NS). STZ resulted in diabetic neuropathy with hyperglycemia and a lower paw withdrawal threshold (PWT), accompanied by elevations in the expression of the NADPH oxidase subunits p47(phox) and gp91(phox) and in the levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA) and a reduction in superoxide dismutase (SOD) activity (P<0.05) in the spinal cord. Both curcumin and apocynin ameliorated diabetic neuropathy. In conclusion, curcumin attenuated neuropathic pain in diabetic rats, at least partly by inhibiting NADPH oxidase-mediating oxidative stress in the spinal cord.
Palavras-chave
Apocynin; Curcumin; Diabetes; H(2)O(2); MDA; NADPH; NADPH oxidase; NOX2; NS; Neuropathy; ROS; SOD; STZ; Spinal cord; hydrogen peroxide; malondialdehyde; nicotinamide adenine dinucleotide phosphate; nicotinamide adenine dinucleotide phosphate oxidase 2; normal saline; reactive oxygen species; streptozotocin; superoxide dismutase
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Medula Espinal
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Estresse Oxidativo
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NADPH Oxidases
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Curcumina
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Neuropatias Diabéticas
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Antioxidantes
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article