Highly flexible UV-vis radiation sources and novel detection schemes for spectrophotometric HPLC detection.
Anal Chem
; 86(2): 1146-52, 2014 Jan 21.
Article
em En
| MEDLINE
| ID: mdl-24377323
ABSTRACT
The concept and performance of the first multiwavelength deep UV light-emitting-diode-based high-performance liquid chromatography (HPLC) absorbance detector are presented. In single-wavelength mode and with optical reference, the limit of detection (LOD) is comparable to conventional state-of-the-art HPLC absorbance detectors. In multiwavelength mode--at present up to eight wavelengths without optical reference--the LOD is about 10 times higher than in single-wavelength mode. Multiplexing and demultiplexing methods are used to separate chromatographic signals in multiwavelength mode and keeps the detector configuration simple and yet flexible. Depending on the operation mode, stray light is either totally negligible or controlled electronically and digitally.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tolueno
/
Cafeína
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Cromatografia Líquida de Alta Pressão
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Antracenos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article