[Determination of taxifolin in Polygonum orientale of different storage period].
Zhongguo Zhong Yao Za Zhi
; 38(17): 2779-81, 2013 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-24380297
ABSTRACT
OBJECTIVE:
To find out the correlation between the content of taxifolin in Polygonum orientale and the storage time.METHOD:
HPLC was used to determine taxifolin. The chromatographic condition was as following Diamonsil C18 column (4.6 mm x 200 mm, 5 microm), mobile phase acetonitrile -0.1% phosphoric acid (gradient elution), the detection wavelength 290 nm and flow rate 1.0 mL x min(-1), the column temperature 30 degrees C.RESULT:
The injection volume of taxifolin was in good linearity within 0.07 and 0.35 microg, the average recovery was 99.7% with RSD 0.2%. Taxifolin content was 0.84, 1.36, 1.75, 1.99 mg x g(-1) corresponding to storage time of 10, 7, 6, 5 years, respectively.CONCLUSION:
The content of taxifolin decreased with the storage time. When the storage period is more than six years, the content is lower than that required by Chinese Pharmacopoeia (2010 version). This method has a good repeatability and accuracy, it provides a scientific reference for clinical use and quality evaluation of P. orientale.
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Base de dados:
MEDLINE
Assunto principal:
Quercetina
/
Medicamentos de Ervas Chinesas
/
Polygonum
/
Armazenamento de Medicamentos
Tipo de estudo:
Evaluation_studies
Idioma:
Zh
Ano de publicação:
2013
Tipo de documento:
Article