Application of a modified EDTA-mediated exudation technique and guttation fluid analysis for Potato spindle tuber viroid RNA detection in tomato plants (Solanum lycopersicum).

Kovalskaya, Natalia; Owens, Robert; Baker, C Jacyn; Deahl, Kenneth; Hammond, Rosemarie W

Kovalskaya, Natalia; Owens, Robert; Baker, C Jacyn; Deahl, Kenneth; Hammond, Rosemarie W.

Afiliação

Kovalskaya N; Molecular Plant Pathology Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA; Institute of Ecological Soil Science of MV Lomonosov Moscow State University, Moscow 119991, Russia.
Owens R; Molecular Plant Pathology Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA.
Baker CJ; Molecular Plant Pathology Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA.
Deahl K; Genetic Improvement of Fruits and Vegetables Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA.
Hammond RW; Molecular Plant Pathology Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA. Electronic address: rose.hammond@ars.usda.gov.

J Virol Methods ; 198: 75-81, 2014 Mar.

Article em En | MEDLINE | ID: mdl-24388932

Assuntos

Ácido Edético/química; Doenças das Plantas/genética; RNA/genética; Solanum lycopersicum/virologia; Viroides/genética; Floema/virologia; Folhas de Planta/virologia; RNA Circular

Palavras-chave

EDTA-mediated phloem exudation; Guttation fluid; Potato spindle tuber viroid; Solanum lycopersicum

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Viroides / RNA / Ácido Edético / Solanum lycopersicum Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2014 Tipo de documento: Article

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