ent-kaurane and ent-pimarane diterpenes from Siegesbeckia pubescens inhibit lipopolysaccharide-induced nitric oxide production in BV2 microglia.
Biol Pharm Bull
; 37(1): 152-7, 2014.
Article
em En
| MEDLINE
| ID: mdl-24389489
ABSTRACT
The extract of Siegesbeckia pubescens herb and its chemical constituents were tested for the ability to inhibit lipopolysaccharide (LPS)-induced nitric oxide (NO) production in BV2 microglia. The methanol extract and the 90% MeOH fraction of S. pubescens effectively attenuated lipopolysaccharide-induced nitric oxide production. Several steps of chromatography yielded eight ent-kaurane diterpenes (1-8) and one ent-pimarane diterpene (9) from the 90% MeOH fraction. Among these compounds, compounds 2-9 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglia. Compounds 3 and 9 concentration-dependently decreased the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), supported by quantitative real time polymerase chain reaction (PCR) and Western blot analysis. These results suggest that ent-kaurane and ent-pimarane diterpenes isolated from S. pubescens are expected to be potential candidates against neuroinflammation-related disease.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Microglia
/
Asteraceae
/
Abietanos
/
Diterpenos do Tipo Caurano
/
Inflamação
/
Fitoterapia
/
Óxido Nítrico
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article