Molecular cloning and expression of IRAK-4, IL-17 and I-κB genes in Haliotis rufescens challenged with Vibrio anguillarum.
Fish Shellfish Immunol
; 36(2): 503-9, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-24398261
ABSTRACT
The candidate genes interleukin-1 receptor associated kinase 4 (IRAK-4), Interleukin 17 (IL-17) and Inhibitor of NF-κB (I-κB) were cloned and evaluated in Californian abalone (Haliotis rufescens) hemocytes in response to Vibrio anguillarum. Molecular characterization evidenced that HrI-κB has a full cDNA sequence of 3027 bp with an encoding region of 401 amino acids (aa), HrIRAK-4 comprised 1969 bp that encoded for 516 aa, and Hr-IL17 had a full sequence of 806 bp encoding for 165 aa. qPCR analysis showed the higher constitutive expression level of Hr-IL17 in hemocytes; meanwhile Hr-IκB and Hr-IRAK4 gene expression levels were higher in gills and mantle. The assessment of gene expression in hemocytes after infection with V. anguillarum evidences the immune responses of Hr-IκB, Hr-IRAK4, and Hr-IL17 and their relationships through the NF-κB signaling pathway.
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Base de dados:
MEDLINE
Assunto principal:
Caramujos
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Regulação da Expressão Gênica
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Interleucina-17
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Proteínas I-kappa B
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Quinases Associadas a Receptores de Interleucina-1
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Imunidade Inata
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article