Generation and characterization of potential dengue vaccine candidates based on domain III of the envelope protein and the capsid protein of the four serotypes of dengue virus.
Arch Virol
; 159(7): 1629-40, 2014 Jul.
Article
em En
| MEDLINE
| ID: mdl-24420159
ABSTRACT
Dengue is currently one of the most important arthropod-borne diseases, causing up to 25,000 deaths annually. There is currently no vaccine to prevent dengue virus infection, which needs a tetravalent vaccine approach. In this work, we describe the cloning and expression in Escherichia coli of envelope domain III-capsid chimeric proteins (DIIIC) of the four dengue serotypes as a tetravalent dengue vaccine candidate that is potentially able to generate humoral and cellular immunity. The recombinant proteins were purified to more than 85 % purity and were recognized by anti-dengue mouse and human sera. Mass spectrometry analysis verified the identity of the proteins and the correct formation of the intracatenary disulfide bond in the domain III region. The chimeric DIIIC proteins were also serotype-specific, and in the presence of oligonucleotides, they formed aggregates that were visible by electron microscopy. These results support the future use of DIIIC recombinant chimeric proteins in preclinical studies in mice for assessing their immunogenicity and efficacy.
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Base de dados:
MEDLINE
Assunto principal:
Regulação Viral da Expressão Gênica
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Proteínas do Envelope Viral
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Vírus da Dengue
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Proteínas do Capsídeo
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Vacinas contra Dengue
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article