Inhibitory role of Id1 on TGF-ß-induced collagen expression in human dermal fibroblasts.
Biochem Biophys Res Commun
; 444(1): 81-5, 2014 Jan 31.
Article
em En
| MEDLINE
| ID: mdl-24434151
Inhibitor of DNA binding 1 (Id1) is a basic helix-loop-helix (bHLH) protein that has a variety of functional roles in cellular events including differentiation, cell cycle and cancer development. In addition, it has been demonstrated that Id1 is related with TGF-ß and Smad signaling in various biological conditions. In this study, we investigated the effect of Id1 on TGF-ß-induced collagen expression in human dermal fibroblasts. When Id1-b isoform was overexpressed, TGF-ß-induced collagen expression was markedly inhibited. Consistent with this result, Id1-b significantly inhibited TGF-ß-induced collagen gel contraction. In addition, Id1-b inhibited TGF-ß-induced phosphorylation of Smad2 and Smad3. Finally, immunohistochemistry showed that Id1 expression was decreased in fibrotic skin diseases while TGF-ß signaling was increased. Together, these results suggest that Id1 is an inhibitory regulator on TGF-ß-induced collagen expression in dermal fibroblasts.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pele
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Fator de Crescimento Transformador beta
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Colágeno Tipo I
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Proteína 1 Inibidora de Diferenciação
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article