Identification of the high molecular weight isoform of phostensin.
Int J Mol Sci
; 15(1): 1068-79, 2014 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-24434620
ABSTRACT
Phostensin is encoded by KIAA1949. 5'-RACEanalysis has been used to identify the translation start site of phostensin mRNA, indicating that it encodes 165 amino acids with an apparent molecular weight of 26 kDa on SDS-PAGE. This low-molecular-weight phostensin is present in human peripheral blood mononuclear cells and many leukemic cell lines. Phostensin is a protein phosphatase-1(PP1) binding protein. It also contains one actin-binding motif at its C-terminal region and binds to the pointed ends of actin filaments, modulating actin dynamics. In the current study, a high-molecular-weight phostensin is identified by using immunoprecipitationin combination with a proteomic approach. This new species of phostensin is also encoded by KIAA1949 and consists of 613 amino acids with an apparent molecular weight of 110 kDa on SDS-PAGE. The low-molecular-weight and high-molecular-weight phostensins were named as phostensin-α and phostensin-ß, respectively. Although phostensin-α is the C-terminal region of phostensin-ß, it is not degraded from phostensin-ß. Phostensin-ß is capable of associating with PP1 and actin filaments, and is present in many cell lines.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteína Fosfatase 1
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article