Validation of a liquid chromatography-electrospray ionization-tandem mass spectrometry method for determination of all-trans retinoic acid in human plasma and its application to a bioequivalence study.
Molecules
; 19(1): 1189-200, 2014 Jan 17.
Article
em En
| MEDLINE
| ID: mdl-24445345
ABSTRACT
A sensitive, reliable and specific LC-MS-MS method was developed and validated for the identification and quantitation of all-trans retinoic acid (ATRA) in human plasma. Acitretin was used as the internal standard (IS). After liquid-liquid extraction of 500 µL plasma with methyl tert-butyl ether (MTBE), ATRA and the IS were chromatographed on a HyPURITY C18 column (150 mm×2.1 mm, 5 µm) with the column temperature set at 40 °C. The mobile phase was consisted of 40% phase A (MTBE-methanol-acetic acid, 50500.5, v/v) and 60% phase B (water-methanol-acetic acid, 50500.5, v/v) with a flow rate of 0.3 mL/min. The API 4000 triple quadrupole mass spectrometer was operated in multiple reaction monitoring (MRM) mode via the positive electrospray ionization interface using the transition m/z 301.4â123.1 for ATRA and m/z 326.9â177.1 for IS, respectively. The calibration curve was linear over the range of 0.45-217.00 ng/mL (r≥0.999) with a lower limit of quantitation (LLOQ) of 0.45 ng/mL. The intra- and inter-day precisions values were below 8% relative standard deviation and the accuracy was from 98.98% to 106.19% in terms of relative error. The validated method was successfully applied in a bioequivalence study of ATRA in Chinese healthy volunteers.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tretinoína
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Espectrometria de Massas por Ionização por Electrospray
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Antineoplásicos
Limite:
Humans
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Male
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article