A new colorimetric strategy for monitoring caspase 3 activity by HRP-mimicking DNAzyme-peptide conjugates.
Analyst
; 139(5): 1178-83, 2014 Mar 07.
Article
em En
| MEDLINE
| ID: mdl-24445423
ABSTRACT
A new method for caspase 3 activity assay has been developed based on HRP-mimicking DNAzyme-peptide conjugates. The mechanism of detection was based on the specific cleavage of DEVD-peptides by active caspase 3 for recognition and the catalytic properties of HRP-mimicking DNAzymes for signal amplification. Under optimal conditions, the detection limit of caspase 3 was 0.89 nM. The proposed method was also successfully applied for the detection of caspase 3 in apoptosis cell lysates.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
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Técnicas Biossensoriais
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DNA Catalítico
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Caspase 3
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Peroxidase do Rábano Silvestre
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article