An insight into the skin penetration enhancement mechanism of N-methylpyrrolidone.
Mol Pharm
; 11(3): 1014-21, 2014 Mar 03.
Article
em En
| MEDLINE
| ID: mdl-24446970
ABSTRACT
This work aims to elucidate the mechanism by which N-methylpyrrolidone (NMP) enhances the skin permeation of a compound by combining experimental data with molecular dynamic (MD) simulations. The addition of 10% NMP significantly increased the propranolol (PR) permeation through the human epidermis (â¼ 15 µg/cm(2) vs â¼ 30 µg/cm(2)) while resulting inefficacious on hydrocortisone (HC) diffusion. No significant alterations in the stratum corneum structure were found after the in vitro treatment of human epidermis with NMP dispersed in mineral oil or water by attenuated total reflectance Fourier transform infrared (ATR-FTIR) analyses. MD simulations revealed the formation of a complex by H-bonds and the π-π stacking interactions between the NMP's amido group and the drug's aromatic systems. The size of the depicted NMP/PR clusters was in line with the hydrodynamic radius derived by dynamic light scattering analyses (â¼ 2.00 nm). Conversely, no interaction, and consequently cluster formation, between NMP and HC occurred. These results suggest that NMP is effective in enhancing the drug permeation through human epidermis by a cotransport mechanism when NMP/drug interaction occurs.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Propranolol
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Pirrolidinonas
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Pele
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Absorção Cutânea
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Hidrocortisona
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Permeabilidade da Membrana Celular
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Sistemas de Liberação de Medicamentos
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article