[Effect of integrin ß8 on TGF-ß1 activation in astrocytes with oxygen glucose deprivation].
Zhongguo Dang Dai Er Ke Za Zhi
; 16(1): 73-6, 2014 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-24461183
ABSTRACT
OBJECTIVE:
To study the effect of ß8 expression on transforming growth factor ß1(TGF-ß1) activation in astrocytes with oxygen glucose deprivation (OGD).METHODS:
Astrocytes were cultured and then subjected to OGD to generate hypoxia-ischemia (HI) model in vitro. Immunocytochemistry was used to detect the expression and distribution of ß8 in nomoxia cultured cells. ß8 protein expression was quantified by Western blot at 12 hours, 1 day and 2 days after OGD. Astrocytes and luciferase reporter cells (TMLC) were co-cultured. ß8 RNA interference system was established to specifically inhibit ß8 expression in cultured astrocytes. TGF-ß1 activation was then detected in the co-culture system.RESULTS:
ß8 was mainly located in the cytoplasm and neurites of astrocytes. OGD resulted in increase of ß8 protein expression at 12 hours after reoxygenation in astrocytes, which was peaked at 1 day after reoxygenation. TGF-ß1 activation was in accordance with ß8 expression in astrocyte-TMLC co-culture system after reoxygenation. After the inhibition of ß8, TGF-ß1 activation was significantly reduced in all time points.CONCLUSIONS:
The highly expressed ß8 plays important roles in the regulation of TGF-ß1 activation in neonatal rats with hypoxic-ischemic brain damage.
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Base de dados:
MEDLINE
Assunto principal:
Astrócitos
/
Hipóxia-Isquemia Encefálica
/
Cadeias beta de Integrinas
/
Fator de Crescimento Transformador beta1
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2014
Tipo de documento:
Article