Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores.
EMBO Rep
; 15(3): 291-8, 2014 Mar.
Article
em En
| MEDLINE
| ID: mdl-24477934
ABSTRACT
The spindle assembly checkpoint inhibits anaphase until all chromosomes have become attached to the mitotic spindle. A complex between the checkpoint proteins Mad1 and Mad2 provides a platform for Mad2Mad2 dimerization at unattached kinetochores, which enables Mad2 to delay anaphase. Here, we show that mutations in Bub1 and within the Mad1 C-terminal domain impair the kinetochore localization of Mad1Mad2 and abrogate checkpoint activity. Artificial kinetochore recruitment of Mad1 in these mutants co-recruits Mad2; however, the checkpoint remains non-functional. We identify specific mutations within the C-terminal head of Mad1 that impair checkpoint activity without affecting the kinetochore localization of Bub1, Mad1 or Mad2. Hence, Mad1 potentially in conjunction with Bub1 has a crucial role in checkpoint signalling in addition to presenting Mad2.
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Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
/
Proteínas Nucleares
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Cinetocoros
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Proteínas de Ciclo Celular
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Proteínas de Schizosaccharomyces pombe
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Pontos de Checagem da Fase M do Ciclo Celular
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Proteínas Mad2
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article