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Clinical epidemiology of bocavirus, rhinovirus, two polyomaviruses and four coronaviruses in HIV-infected and HIV-uninfected South African children.
Nunes, Marta C; Kuschner, Zachary; Rabede, Zelda; Madimabe, Richard; Van Niekerk, Nadia; Moloi, Jackie; Kuwanda, Locadiah; Rossen, John W; Klugman, Keith P; Adrian, Peter V; Madhi, Shabir A.
Afiliação
  • Nunes MC; Department of Science and Technology/National Research Foundation: Vaccine Preventable Diseases, Johannesburg, South Africa ; Medical Research Council: Respiratory and Meningeal Pathogens Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
  • Kuschner Z; Stony Brook School of Medicine, Stony Brook University, Stony Brook, New York, United States of America.
  • Rabede Z; Medical Research Council: Respiratory and Meningeal Pathogens Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
  • Madimabe R; Department of Science and Technology/National Research Foundation: Vaccine Preventable Diseases, Johannesburg, South Africa ; Medical Research Council: Respiratory and Meningeal Pathogens Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
  • Van Niekerk N; Department of Science and Technology/National Research Foundation: Vaccine Preventable Diseases, Johannesburg, South Africa ; Medical Research Council: Respiratory and Meningeal Pathogens Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
  • Moloi J; Molecular and Immunology Division, BioMérieux, Johannesburg, South Africa.
  • Kuwanda L; Department of Science and Technology/National Research Foundation: Vaccine Preventable Diseases, Johannesburg, South Africa ; Medical Research Council: Respiratory and Meningeal Pathogens Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
  • Rossen JW; Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
  • Klugman KP; Medical Research Council: Respiratory and Meningeal Pathogens Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa ; Hubert Department of Global Health, Rollins School of Public Health and Division of Infectious Diseases, Emory University School of M
  • Adrian PV; Department of Science and Technology/National Research Foundation: Vaccine Preventable Diseases, Johannesburg, South Africa ; Medical Research Council: Respiratory and Meningeal Pathogens Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
  • Madhi SA; Department of Science and Technology/National Research Foundation: Vaccine Preventable Diseases, Johannesburg, South Africa ; Medical Research Council: Respiratory and Meningeal Pathogens Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa ; Nationa
PLoS One ; 9(2): e86448, 2014.
Article em En | MEDLINE | ID: mdl-24498274
BACKGROUND: Advances in molecular diagnostics have implicated newly-discovered respiratory viruses in the pathogenesis of pneumonia. We aimed to determine the prevalence and clinical characteristics of human bocavirus (hBoV), human rhinovirus (hRV), polyomavirus-WU (WUPyV) and -KI (KIPyV) and human coronaviruses (CoV)-OC43, -NL63, -HKU1 and -229E among children hospitalized with lower respiratory tract infections (LRTI). METHODS: Multiplex real-time reverse-transcriptase polymerase chain reaction was undertaken on archived nasopharyngeal aspirates from HIV-infected and -uninfected children (<2 years age) hospitalized for LRTI, who had been previously investigated for respiratory syncytial virus, human metapneumovirus, parainfluenza I-III, adenovirus and influenza A/B. RESULTS: At least one of these viruses were identified in 274 (53.0%) of 517 and in 509 (54.0%) of 943 LRTI-episodes in HIV-infected and -uninfected children, respectively. Human rhinovirus was the most prevalent in HIV-infected (31.7%) and -uninfected children (32.0%), followed by CoV-OC43 (12.2%) and hBoV (9.5%) in HIV-infected; and by hBoV (13.3%) and WUPyV (11.9%) in HIV-uninfected children. Polyomavirus-KI (8.9% vs. 4.8%; p = 0.002) and CoV-OC43 (12.2% vs. 3.6%; p<0.001) were more prevalent in HIV-infected than -uninfected children. Combined with previously-tested viruses, respiratory viruses were identified in 60.9% of HIV-infected and 78.3% of HIV-uninfected children. The newly tested viruses were detected at high frequency in association with other respiratory viruses, including previously-investigated viruses (22.8% in HIV-infected and 28.5% in HIV-uninfected children). CONCLUSIONS: We established that combined with previously-investigated viruses, at least one respiratory virus was identified in the majority of HIV-infected and HIV-uninfected children hospitalized for LRTI. The high frequency of viral co-infections illustrates the complexities in attributing causality to specific viruses in the aetiology of LRTI and may indicate a synergetic role of viral co-infections in the pathogenesis of childhood LRTI.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções Respiratórias / Viroses / Infecções por HIV / Coinfecção Tipo de estudo: Prevalence_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Limite: Child, preschool / Humans / Infant País como assunto: Africa Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções Respiratórias / Viroses / Infecções por HIV / Coinfecção Tipo de estudo: Prevalence_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Limite: Child, preschool / Humans / Infant País como assunto: Africa Idioma: En Ano de publicação: 2014 Tipo de documento: Article