Tumor-homing glycol chitosan-based optical/PET dual imaging nanoprobe for cancer diagnosis.
Bioconjug Chem
; 25(3): 601-10, 2014 Mar 19.
Article
em En
| MEDLINE
| ID: mdl-24506683
ABSTRACT
Imaging techniques including computed tomography, magnetic resonance imaging, and positron emission tomography (PET) offer many potential benefits to diagnosis and treatment of cancers. Each method has its own strong and weak points. Therefore, multimodal imaging techniques have been highlighted as an alternative method for overcoming the limitations of each respective imaging method. In this study, we fabricated PET/optical activatable imaging probe based on glycol chitosan nanoparticles (CNPs) for multimodal imaging. To prepare the dual PET/optical probes based on CNPs, both (64)Cu radiolabeled DOTA complex and activatable matrix metalloproteinase (MMP)-sensitive peptide were chemically conjugated onto azide-functionalized CNPs via bio-orthogonal click chemistry, which was a reaction between azide group and dibenzyl cyclooctyne. The PET/optical activatable imaging probes were visualized by PET and optical imaging system. Biodistribution of probes and activity of MMP were successfully measured in tumor-bearing mice.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Compostos Radiofarmacêuticos
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Nanotecnologia
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Tomografia por Emissão de Pósitrons
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Nanopartículas
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Dispositivos Ópticos
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Neoplasias Experimentais
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
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Humans
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Male
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article