Coarse-grained simulations of the HIV-1 matrix protein anchoring: revisiting its assembly on membrane domains.
Biophys J
; 106(3): 577-85, 2014 Feb 04.
Article
em En
| MEDLINE
| ID: mdl-24507598
ABSTRACT
In the accepted model for human immunodeficiency virus preassembly in infected host cells, the anchoring to the intracellular leaflet of the membrane of the matrix domain (MA) that lies at the N-terminus of the viral Gag protein precursor appears to be one of the crucial steps for particle assembly. In this study, we simulated the membrane anchoring of human immunodeficiency virus-1 myristoylated MA protein using a coarse-grained representation of both the protein and the membrane. Our calculations first suggest that the myristoyl group could spontaneously release from its initial hydrophobic pocket before MA protein interacts with the lipid membrane. All-atom simulations confirmed this possibility with a related energy cost estimated to be ~5 kcal.mol(-1). The phosphatidylinositol (4,5) bisphosphate (PI(4,5)P2) head binds preferentially to the MA highly basic region as described in available NMR data, but interestingly without flipping of its 2' acyl chain into the MA protein. Moreover, MA was able to confine PI(4,5)P2 lipids all around its molecular surface after having found a stable orientation at the membrane surface. Our results suggest that this orientation is dependent on Myr anchoring and that this confinement induces a lateral segregation of PI(4,5)P2 in domains. This is consistent with a PI(4,5)P2 enrichment of the virus envelope as compared to the host cell membrane.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Antígenos HIV
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Fosfatidilinositol 4,5-Difosfato
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Produtos do Gene gag do Vírus da Imunodeficiência Humana
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Simulação de Dinâmica Molecular
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article