Pyrosequencing: powerful and quantitative sequencing technology.
Curr Protoc Mol Biol
; 104: 7.15.1-7.15.23, 2013 Oct 11.
Article
em En
| MEDLINE
| ID: mdl-24510299
ABSTRACT
Pyrosequencing is a sequencing-by-synthesis method for DNA analysis that has emerged as a platform not only for de novo sequencing applications, but also for quantitative analysis of genomic methylation, single-nucleotide polymorphisms, and allele quantification. In this unit, we describe a complete workflow from sample to result that is suitable for each of these applications. As cytosine conversion is a key element of successful methylation analysis using pyrosequencing, a support protocol for bisulfite treatment is also included.
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Base de dados:
MEDLINE
Assunto principal:
Análise de Sequência de DNA
Tipo de estudo:
Guideline
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article